il 6 goat polyclonal igg  (Santa Cruz Biotechnology)

Journal: Frontiers in Immunology

Article Title: Stage of Gestation at Porcine Epidemic Diarrhea Virus Infection of Pregnant Swine Impacts Maternal Immunity and Lactogenic Immune Protection of Neonatal Suckling Piglets

doi: 10.3389/fimmu.2019.00727

Figure Lengend Snippet: Overall summary of results and significance for the major immune and PEDV protection parameters assessed.

Article Snippet: Biotinylated anti-porcine IL-4 (0.5 μg/ml, clone A155B15C6), anti-porcine IL-10 (1 μg/ml, clone 945A1A926C2), anti-porcine IFN-γ (0.5 μg/ml, clone A151D13C5), anti-TGF-β (0.4 μg/ml, TGF-β-1 Multispecies Ab Pair CHC1683) (Thermo Fisher Scientific, Waltham, MA), anti-porcine IL-6 (0.1 μg/ml, goat polyclonal IgG), anti-porcine IL-12 (0.2 μg/ml, goat polyclonal IgG), anti-porcine IFN-α (3.75 μg/ml, clone F17) (R&D systems, Minneapolis, MN), anti-porcine TNF-α (0.4 μg/ml, goat polyclonal Ab), anti-porcine IL-17 (1 μg/ml, rabbit polyclonal Ab) or anti-porcine IL-22 (1 μg/ml, rabbit polyclonal Ab) (Kingfisher Biotech, Saint Paul, MN) were used for detection.

Techniques: Activity Assay, Concentration Assay, Activation Assay, Virus, Neutralization

Journal: Frontiers in Immunology

Article Title: Stage of Gestation at Porcine Epidemic Diarrhea Virus Infection of Pregnant Swine Impacts Maternal Immunity and Lactogenic Immune Protection of Neonatal Suckling Piglets

doi: 10.3389/fimmu.2019.00727

Figure Lengend Snippet: Peripheral natural killer (NK) cell frequency and function and serum interleukin (IL)-12 and IL-22 concentrations were increased in first compared with second and third trimester gilts. (A) Peripheral blood mononuclear cells (PBMCs) were isolated and NK cell (CD3 − , CD172 − , CD8 + ) frequencies were determined by flow cytometry at post infection day (PID) 0, 6–8, and 12–17. (B) PBMCs and CFSE-stained K562 tumor cells were used as effector and target cells, respectively, and co-cultured at 10:1 5:1 and 1:1 ratios to assess NK cell cytotoxic function at PID 6–8. (C) Serum cytokine concentrations (pg/ml) of IL-12 and (D) IL-22 were measured at PID 0, 6–8, and 12–17. Asterisks indicate significant differences among treatment groups (mean ± SEM). Statistical analysis was performed using the Student's t -test (B) or two-way ANOVA with repeated measures and Bonferroni's correction for multiple comparisons (A,C,D) . * P < 0.05, ** P < 0.01.

Article Snippet: Biotinylated anti-porcine IL-4 (0.5 μg/ml, clone A155B15C6), anti-porcine IL-10 (1 μg/ml, clone 945A1A926C2), anti-porcine IFN-γ (0.5 μg/ml, clone A151D13C5), anti-TGF-β (0.4 μg/ml, TGF-β-1 Multispecies Ab Pair CHC1683) (Thermo Fisher Scientific, Waltham, MA), anti-porcine IL-6 (0.1 μg/ml, goat polyclonal IgG), anti-porcine IL-12 (0.2 μg/ml, goat polyclonal IgG), anti-porcine IFN-α (3.75 μg/ml, clone F17) (R&D systems, Minneapolis, MN), anti-porcine TNF-α (0.4 μg/ml, goat polyclonal Ab), anti-porcine IL-17 (1 μg/ml, rabbit polyclonal Ab) or anti-porcine IL-22 (1 μg/ml, rabbit polyclonal Ab) (Kingfisher Biotech, Saint Paul, MN) were used for detection.

Techniques: Isolation, Flow Cytometry, Infection, Staining, Cell Culture

Journal: Frontiers in Immunology

Article Title: Stage of Gestation at Porcine Epidemic Diarrhea Virus Infection of Pregnant Swine Impacts Maternal Immunity and Lactogenic Immune Protection of Neonatal Suckling Piglets

doi: 10.3389/fimmu.2019.00727

Figure Lengend Snippet: Second trimester gilts had significantly increased circulating PEDV specific IgA and IgG antibody secreting cells (ASCs) at post infection day (PID) 12–17, PEDV specific IgA antibodies (Abs) at PID 6–8 and concentrations of transforming growth factor (TGF)-β at PID 0 compared with first and third trimester gilts. (A) Peripheral blood mononuclear cells (PBMCs) were isolated and added to PEDV ELISPOT plates to determine the PEDV specific IgA and (B) IgG ASCs. (C) Serum PEDV IgA Ab titers and cytokine concentrations (pg/ml) of (D) TGF-β and (E) interleukin (IL)-6 were determined by ELISA. Gilts were sampled at PID 0, 6–8, and 12–17. Asterisks indicate significant differences among treatment groups at the same time point (mean ± SEM). Statistical analysis was performed using the two-way ANOVA with repeated measures and Bonferroni's correction for multiple comparisons. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: Biotinylated anti-porcine IL-4 (0.5 μg/ml, clone A155B15C6), anti-porcine IL-10 (1 μg/ml, clone 945A1A926C2), anti-porcine IFN-γ (0.5 μg/ml, clone A151D13C5), anti-TGF-β (0.4 μg/ml, TGF-β-1 Multispecies Ab Pair CHC1683) (Thermo Fisher Scientific, Waltham, MA), anti-porcine IL-6 (0.1 μg/ml, goat polyclonal IgG), anti-porcine IL-12 (0.2 μg/ml, goat polyclonal IgG), anti-porcine IFN-α (3.75 μg/ml, clone F17) (R&D systems, Minneapolis, MN), anti-porcine TNF-α (0.4 μg/ml, goat polyclonal Ab), anti-porcine IL-17 (1 μg/ml, rabbit polyclonal Ab) or anti-porcine IL-22 (1 μg/ml, rabbit polyclonal Ab) (Kingfisher Biotech, Saint Paul, MN) were used for detection.

Techniques: Infection, Isolation, Enzyme-linked Immunospot, Enzyme-linked Immunosorbent Assay

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Non-canonical STAT3 activation regulates excess TGF-β1 and Collagen I expression in muscle of stricturing Crohn's disease

doi: 10.4049/jimmunol.1401779

Figure Lengend Snippet: Table 2

Article Snippet: Human IL-6 polyclonal goat IgG (R&D systems Inc, Minneapolis, MN), Collagen I, α-smooth muscle actin (α-SMA, Sigma-aldrich Inc, St. Louis, MO), phosphorylated STAT3(S727) and phosphorylated STAT3(Y705) were examined by immunofluorescence using specific antibodies (Cell Signal Technology, Beverly, MA) and Alexa Fluor 594- and 488-conjugated secondary antibodies (Molecular Probes, Eugene, OR).

Techniques: